Effect of some pesticides on growth, nitrogen fixation and nifgenes in Azotobacter chroococcum and Azotobacter vinelandii isolated from soil

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Nitrogen Fixation by Azotobacter vinelandii

Nitrogenase was isolated and purified from wildtype and a tungsten-resistant mutant (LM2) of Azotobacter vinelandii strain OP derepressed on medium containing 1-10 mM W. While the enzyme from the wild-type strain contained the polypeptides of the conventional enzyme, metal analysis of component 1 demonstrated the existence of one atom each of molybdenum and tungsten. Furthermore, the ESR spectr...

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Symbiosis between Chlorella Sp. and Azotobacter Chroococcum and Nitrogen Fixation

Alfred Koch has given considerable attention to the subject of nitrogen fixation by bacteria as resulting from their symbiosis with alga~. Many others have also investigated various features of this problem. In spite of all this there are numerous questions which remain unsolved. The use of soil cultures for such studies is objectionable on account of the soil's complexity, its variety of flora...

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Evidence for an alternative nitrogen fixation system in Azotobacter vinelandii.

Two Azotobacter vinelandii strains capable of growing on N2(Nif+) were isolated from two different mutant strains that lacked dinitrogenase activity (Nif-). Extracts of N2-grown cells of the two Nif+ strains lacked significant amounts of the "conventional" dinitrogenase protein subunits, as determined by two-dimensional gel electrophoresis. Instead, the extracts contained at least four new prot...

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Evaluation of three methods for preservation of Azotobacter chroococcum and Azotobacter vinelandii

Plant growth-promoting bacteria –PGPB– are microorganisms that can grow in, on, or around plant tissues and stimulate plant growth by numerous mechanisms (Vessey 2003). Within this group, nitrogen-fixing bacteria play a remarkable role in plant nutritition. They can take N2 from atmosphere and make it available for plant uptake (Halbleib and Ludden 2000). Azotobacter, a nitrogen-fixing bacteria...

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Oxidative activity and nitrogen fixation in cell-free preparations from Azotobacter vinelandii.

Ammonia is generally conceded to be the "key intermediate" in biological nitrogen fixation-that is, the compound which reacts to form the first stable organic compound, glutamic acid (Wilson and Burris, 1953). However, there is virtually no information concerning the reactions functioning in the conversion of N2 to ammonia. Reproducible nitrogen fixation by a cell-free preparation from bacteria...

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ژورنال

عنوان ژورنال: Journal of Toxicology and Environmental Health Sciences

سال: 2013

ISSN: 2006-9820

DOI: 10.5897/jtehs12.029